Comparative efficiency of accessible transfection methods in model cell lines for biotechnological applications

Transient gene expression is one of the most common methods in molecular biology, equally relevant for basic research projects and biotechnological industries. Despite existence commercial transfection systems, which afford high efficiency levels reporter genes, expanding such systems to industrial scales often problematic due costs reagents. The well-described cationic calcium-phosphate are accessible ensure reproducible results at much lower costs. This study aimed comparative validation calcium phosphate (polyethylenimine-based) protocols along with commercially available TurboFect reagent mono- cotransfections on a panel commonly used cell lines including HEK293T, Huh7, BHK-21, CHO MRC5. plasmid constructs encoding different fluorescent proteins was measured by flow cytometry. Of all tested methods, afforded highest DNA delivery except BHK21, PEI method turned out be more efficient than transfection, CHO, both showed comparable efficiency.